Patient-derived xenograft mouse models to study the central nervous system and retinal tropism of primary and secondary central nervous system lymphomas (2023)

Isbell , LK , Tschuch , C , Doostkam , S , Waldeck , S , Andrieux , G , Shoumariyeh , K , Lenhard , D , Schaefer , HE , Reinacher , PC , Bartsch , I , Pantic , M , Vinnakota , JM ., Kakkassery, V., Schorb, E., Scherer, F., Frey, A. V., Boerries, M., Illerhaus, G., Duyster, J., ... by Bubnoff, N.(2023).Patient-derived xenograft mouse models to study the central nervous system and retinal tropism of primary and secondary central nervous system lymphomas.Neuropathology and Applied Neurobiology,49(2), [e12899].https://doi.org/10.1111/nan.12899

Isbell, Lisa Christina; Tschuch , Chordula ; Doostkam, Soroush et al. /Patient-derived xenograft mouse models to study the central nervous system and retinal tropism of primary and secondary central nervous system lymphomas. Em:Neuropathology and Applied Neurobiology. 2023; Wow. 49, No. 2.

@article{54bb16c643104baa90a1dd2928410c01,

title = "Patient-derived mouse xenograft models to study the central nervous system and retinal tropism of primary and secondary central nervous system lymphomas",

abstract = “Objectives: How and why lymphoma cells lodge in the central nervous system and in the vitreoretinal compartment in primary diffuse large B-cell lymphoma of the central nervous system remains unknown. Our aim was to create an in vivo model to study the tropism of lymphoma cells into the central nervous system Characterized by immunohistochemistry, flow cytometry and nucleic acid sequencing technology. In reimplantation experiments, we analyzed propagation patterns of orthotopic and heterotopic xenografts and performed RNA sequencing of different involved organs to detect differences at the transcriptome, central nervous system, and primary vitreoretinal lymphoma levels, respectively. Transcriptomic analysis revealed clear signatures for lymphoma cells in the brain compared to the spleen, and a low level of overlap of genes commonly regulated in primary and secondary central nervous system lymphomas. Conclusion: This in vivo tumor model preserves key features of primary and secondary central nervous system lymphoma and can be used to explore critical pathways to the central nervous system and retinal trophism with the aim of finding new targets for new therapeutic approaches.

autor = „Isbell, Cordula Tschuch, Soroush Doostkam, Silvia Waldeck, Geoffroy Andrieux, Khalid Shoumariyeh, Dorothee Lenhard, Schaefer, Reinacher, Peter Christoph, [Janaki Manoja], Vinodh Kakkassery, Elisabeth Schorb, Florian Scherer, Frey, Melanie Boerries, Gerald Illerhaus, Justus Duyster, Julia Schueler, von Bubnoff, Nikolas“

note = "Editor's copyright: {\textcopyright} 2023 The Authors. Neuropathology and Applied Neurobiology, published by John Wiley & Sons Ltd on behalf of the British Neuropathological Society.”,

again = "2023",

month = April,

two = "10.1111/in.12899",

language="English",

volume = "49",

Journal = "Neuropathology and Applied Neurobiology",

issn = „0305-1846“,

number = "2",

}

Isbell , LK , Tschuch , C , Doostkam , S , Waldeck , S , Andrieux , G , Shoumariyeh , K , Lenhard , D , Schaefer , HE , Reinacher , PC , Bartsch , I , Pantic , M , Vinnakota , JM, Kakkassery, V, Schorb, E, Scherer, F, Frey, AV, Boerries, M, Illerhaus, G, Duyster, J, Schueler, J2023, 'Patient-derived xenograft mouse models to study the central nervous system and retinal tropism of primary and secondary central nervous system lymphomas',Neuropathology and Applied Neurobiology, Bd. 49, Nr. 2, e12899.https://doi.org/10.1111/nan.12899

Patient-derived xenograft mouse models to study the central nervous system and retinal tropism of primary and secondary central nervous system lymphomas./Isbell, Lisa Christina; Tschuch, Chordula; Doostkam, Soroush et al.

Em:Neuropathology and Applied Neurobiology, Bd. 49, Nr. 2, e12899, 04.2023.

search result:contribution to the journal›newspaper article›To search›Peer-Review

YOU - DIA

T1 - Patient-derived xenograft mouse models to study the central nervous system and retinal tropism of primary and secondary central nervous system lymphomas

AU – Isbell, Lisa Kristina

AU - Tschuch, Córdula

AU – Doostkam, Soroush

AU - Waldeck, Silvia

AU – Andrieux, Geoffroy

AU - Shoumariyeh, Khalid

AU - Lenhard, Dorothee

AU - Schaefer, Hans Eckart

AU - Reinacher, Peter Christoph

AU - Bartsch, Ingrid

AU - Pantic, Milena

AU – Vinnakota, Janaki Manoja

AU – Kakkassery, Vinodh

AU - Schorb, Elisabeth

AU - Scherer, Florian

AU - Frey, Anna Verena

AU – Boerries, Melanie

AU - Illerhaus, Gerald

AU – Duyster, Justus

AU - Schueler, Julia

AU - von Bubnoff, Nikolas

N1 – Editora Copyright: © 2023 The Authors. Neuropathology and Applied Neurobiology, published by John Wiley & Sons Ltd on behalf of the British Neuropathological Society.

Fiscal year – 2023/4

A1 – 2023/4

N2 - Objectives: How and why lymphoma cells lodge in the central nervous system and in the vitreoretinal compartment in primary diffuse large B-cell lymphoma of the central nervous system remains unknown. Our aim was to create an in vivo model to study the tropism of lymphoma cells towards the central nervous system. Methods: We constructed a mouse xenograft model for patients' own central nervous system lymphomas and characterized xenografts from four patients with primary and four secondary central nervous system lymphomas using immunohistochemistry, flow cytometry, and nucleic acid sequencing technology. In reimplantation experiments, we analyzed propagation patterns of orthotopic and heterotopic xenografts and performed RNA sequencing of different involved organs to detect differences at the transcriptome level. Results: We found that xenografted primary central nervous system lymphoma cells return to the central nervous system and eye after intrasplenic transplantation, mimicking central nervous system and primary vitreoretinal lymphoma pathology, respectively. Transcriptomic analysis revealed clear signatures for lymphoma cells in the brain compared to the spleen, and a low level of overlap of genes commonly regulated in primary and secondary central nervous system lymphomas. Conclusion: This in vivo tumor model preserves key features of primary and secondary central nervous system lymphoma and can be used to explore critical pathways to the central nervous system and retinal trophism with the aim of finding new targets for new therapeutic approaches.

AB - Objectives: It is still unknown how and why lymphoma cells lodge in the central nervous system and in the vitreoretinal compartment in primary diffuse large B-cell lymphoma of the central nervous system. Our aim was to create an in vivo model to study the tropism of lymphoma cells towards the central nervous system. Methods: We constructed a mouse xenograft model for patients' own central nervous system lymphomas and characterized xenografts from four patients with primary and four secondary central nervous system lymphomas using immunohistochemistry, flow cytometry, and nucleic acid sequencing technology. In reimplantation experiments, we analyzed propagation patterns of orthotopic and heterotopic xenografts and performed RNA sequencing of different involved organs to detect differences at the transcriptome level. Results: We found that xenografted primary central nervous system lymphoma cells return to the central nervous system and eye after intrasplenic transplantation, mimicking central nervous system and primary vitreoretinal lymphoma pathology, respectively. Transcriptomic analysis revealed clear signatures for lymphoma cells in the brain compared to the spleen, and a low level of overlap of genes commonly regulated in primary and secondary central nervous system lymphomas. Conclusion: This in vivo tumor model preserves key features of primary and secondary central nervous system lymphoma and can be used to explore critical pathways to the central nervous system and retinal trophism with the aim of finding new targets for new therapeutic approaches.

UR – http://www.scopus.com/inward/record.url?scp=85153770764&partnerID=8YFLogxK

U2 - 10.1111/inch 12899

DO-10.1111/nan.12899

M3 – Newspaper article

C2 - 36879456

AN - SCOPUS:85153770764

VL - 49

JO – Neuropathology and Applied Neurobiology

JF - Neuropathology and Applied Neurobiology

SN - 0305-1846

IS - 2

M1 - e12899

AND -

Isbell LK, Tschuch C, Doostkam S, Waldeck S, Andrieux G, Shoumariyeh K et al.Patient-derived xenograft mouse models to study the central nervous system and retinal tropism of primary and secondary central nervous system lymphomas.Neuropathology and Applied Neurobiology. 2023 April;49(2):e12899. doi: 10.1111/nan.12899